Unit

UNIT 7.23 Isolation and Functional Analysis of Human Neutrophils

  1. Douglas B. Kuhns1,
  2. Debra A. Long Priel1,
  3. Jessica Chu2,
  4. Kol A. Zarember2

Published Online: 2 NOV 2015

DOI: 10.1002/0471142735.im0723s111

Lab Protocol Title

Current Protocols in Immunology

Current Protocols in Immunology

Additional Information

How to Cite

Kuhns, D.B. , Long Priel, D.A. , Chu, J. , and Zarember, K.A. 2015. Isolation and functional analysis of human neutrophils. Curr. Protoc. Immunol. 111:7.23.1-7.23.16. doi: 10.1002/0471142735.im0723s111

Author Information

  1. 1

    Neutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland

  2. 2

    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 2 NOV 2015
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Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described. © 2015 by John Wiley & Sons, Inc.

Keywords:

  • neutrophil;
  • leukocyte;
  • NOX2;
  • phagocyte;
  • innate immunity
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