UNIT 7.24 Isolation of Human Basophils

  1. Dean D. Metcalfe

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0724s06

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Metcalfe, D. D. 2001. Isolation of Human Basophils. Current Protocols in Immunology. 6:IV:7.24:7.24.1–7.24.4.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1993

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Human basophils are normally the rarest of all circulating leukocytes and thus are among the most difficult of the blood elements to purify. In this unit, basophils are isolated from leukocyte populations, resulting in preparations of cells of which 5% to 50% are basophils and the rest are lymphocytes along with neutrophils and monocytes. This technique is advantageous because of its speed and because it minimizes basophil stimulation and histamine release.