UNIT 7.24 Isolation of Human Basophils
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Metcalfe, D. D. 2001. Isolation of Human Basophils. Current Protocols in Immunology. 6:IV:7.24:7.24.1–7.24.4.
- Published Online: 1 MAY 2001
- Published Print: JUN 1993
Human basophils are normally the rarest of all circulating leukocytes and thus are among the most difficult of the blood elements to purify. In this unit, basophils are isolated from leukocyte populations, resulting in preparations of cells of which 5% to 50% are basophils and the rest are lymphocytes along with neutrophils and monocytes. This technique is advantageous because of its speed and because it minimizes basophil stimulation and histamine release.