UNIT 7.25 Isolation of Tissue Mast Cells

  1. Dean D. Metcalfe

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0725s01

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Metcalfe, D. D. 2001. Isolation of Tissue Mast Cells. Current Protocols in Immunology. 1:IV:7.25:7.25.1–7.25.3.

Author Information

  1. National Institute of Allergy And Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1992

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Mast cells, unlike other immune effector cells, are not found in the circulation and thus must be obtained from accessible tissues such as gastrointestinal and lung tissue. In this unit, a combination of mechanical fragmentation, enzyme digestion, and centrifugation procedures are used to obtain suspensions enriched for mast cells from gastrointestinal tissue. A support protocol details a procedure for acid toluidine blue staining of mast cells.