UNIT 7.25 Isolation of Tissue Mast Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Metcalfe, D. D. 2001. Isolation of Tissue Mast Cells. Current Protocols in Immunology. 1:IV:7.25:7.25.1–7.25.3.
- Published Online: 1 MAY 2001
- Published Print: MAR 1992
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Mast cells, unlike other immune effector cells, are not found in the circulation and thus must be obtained from accessible tissues such as gastrointestinal and lung tissue. In this unit, a combination of mechanical fragmentation, enzyme digestion, and centrifugation procedures are used to obtain suspensions enriched for mast cells from gastrointestinal tissue. A support protocol details a procedure for acid toluidine blue staining of mast cells.