Unit

UNIT 7.25 Isolation of Tissue Mast Cells

  1. Dean D. Metcalfe

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0725s01

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Metcalfe, D. D. 2001. Isolation of Tissue Mast Cells. Current Protocols in Immunology. 1:IV:7.25:7.25.1–7.25.3.

Author Information

  1. National Institute of Allergy And Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1992

This is not the most recent version of the article. View current version (1 AUG 2010)

Abstract

Mast cells, unlike other immune effector cells, are not found in the circulation and thus must be obtained from accessible tissues such as gastrointestinal and lung tissue. In this unit, a combination of mechanical fragmentation, enzyme digestion, and centrifugation procedures are used to obtain suspensions enriched for mast cells from gastrointestinal tissue. A support protocol details a procedure for acid toluidine blue staining of mast cells.