Unit

UNIT 7.31 Isolation of Human Eosinophils

  1. Kaiser G. Lim,
  2. Peter F. Weller

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0731s20

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lim, K. G. and Weller, P. F. 2001. Isolation of Human Eosinophils. Current Protocols in Immunology. 20:IV:7.31:7.31.1–7.31.7.

Author Information

  1. Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1996

This is not the most recent version of the article. View current version (1 AUG 2012)

Abstract

Highly purified eosinophils can be isolated from peripheral blood by negative selection using anti-CD16 immunomagnetic beads. The basic protocol describes a sequential fractionation of peripheral blood in which eosinophils are separated first from mononuclear cells and then from CD16+ neutrophils. This technique is easy to use, fast, and highly reproducible. A support protocol describes a staining method that can be used to evaluate the purity of eosinphils.