UNIT 7.31 Isolation of Human Eosinophils
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Lim, K. G. and Weller, P. F. 2001. Isolation of Human Eosinophils. Current Protocols in Immunology. 20:IV:7.31:7.31.1–7.31.7.
- Published Online: 1 MAY 2001
- Published Print: DEC 1996
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Highly purified eosinophils can be isolated from peripheral blood by negative selection using anti-CD16 immunomagnetic beads. The basic protocol describes a sequential fractionation of peripheral blood in which eosinophils are separated first from mononuclear cells and then from CD16+ neutrophils. This technique is easy to use, fast, and highly reproducible. A support protocol describes a staining method that can be used to evaluate the purity of eosinphils.