UNIT 7.32 Isolation and Generation of Human Dendritic Cells

  1. Thomas F. Tedder,
  2. Paul J. Jansen

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0732s23

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Tedder, T. F. and Jansen, P. J. 2001. Isolation and Generation of Human Dendritic Cells. Current Protocols in Immunology. 23:IV:7.32:7.32.1–7.32.16.

Author Information

  1. Duke University Medical Center, Durham, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1997

This is not the most recent version of the article. View current version (1 NOV 2012)


Dendritic cells are highly specialized antigen-presenting cells (APC), which may be isolated or generated from human blood mononuclear cells. Although mature blood dendritic cells normally represent ˜0.2% of human blood mononuclear cells, their frequency can be greatly increased using the cell enrichment methods described in this unit. More highly purified dendritic cell preparations can be obtained from these populations by sorting of fluorescence-labeled cells. Alternatively, dendritic cells can be generated from monocytes by culture with the appropriate cytokines, as described here. In addition, a negative selection approach is provided that may be employed to generate cell preparations that have been depleted of dendritic cells to be used for comparison in functional studies.