UNIT 7.32 Isolation and Generation of Human Dendritic Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Tedder, T. F. and Jansen, P. J. 2001. Isolation and Generation of Human Dendritic Cells. Current Protocols in Immunology. 23:IV:7.32:7.32.1–7.32.16.
- Published Online: 1 MAY 2001
- Published Print: SEP 1997
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Dendritic cells are highly specialized antigen-presenting cells (APC), which may be isolated or generated from human blood mononuclear cells. Although mature blood dendritic cells normally represent ˜0.2% of human blood mononuclear cells, their frequency can be greatly increased using the cell enrichment methods described in this unit. More highly purified dendritic cell preparations can be obtained from these populations by sorting of fluorescence-labeled cells. Alternatively, dendritic cells can be generated from monocytes by culture with the appropriate cytokines, as described here. In addition, a negative selection approach is provided that may be employed to generate cell preparations that have been depleted of dendritic cells to be used for comparison in functional studies.