Unit

UNIT 7.32 Isolation and Generation of Human Dendritic Cells

  1. Smita Nair,
  2. Gerald E. Archer,
  3. Thomas F. Tedder

Published Online: 1 NOV 2012

DOI: 10.1002/0471142735.im0732s99

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Nair, S., Archer, G. E. and Tedder, T. F. 2012. Isolation and Generation of Human Dendritic Cells. Current Protocols in Immunology. 99:IV:7.32:7.32.1–7.32.23.

Author Information

  1. Duke University Medical Center, Durham, North Carolina

Publication History

  1. Published Online: 1 NOV 2012
  2. Published Print: NOV 2012

Abstract

Dendritic cells are highly specialized antigen-presenting cells (APC), which may be isolated or generated from human blood mononuclear cells. Although mature blood dendritic cells normally represent ∼0.2% of human blood mononuclear cells, their frequency can be greatly increased using the cell enrichment methods described in this unit. More highly purified dendritic cell preparations can be obtained from these populations by sorting of fluorescence-labeled cells. Alternatively, dendritic cells can be generated from monocytes by culture with the appropriate cytokines, as described here. In addition, a negative selection approach is provided that may be employed to generate cell preparations that have been depleted of dendritic cells to be used for comparison in functional studies. Curr. Protoc. Immunol. 99:7.32.1-7.32.23. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • immunology;
  • immunologic study in humans;
  • in vitro assays for immune cell function;
  • immune disease;
  • innate immunity;
  • antigen presentation