Unit

UNIT 7.33 Measurement of Prostaglandins and Other Eicosanoids

  1. William F. Stenson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0733s33

Lab Protocol Title

Current Protocols in Immunology

Current Protocols in Immunology

Additional Information

How to Cite

Stenson, W. F. 2001. Measurement of Prostaglandins and Other Eicosanoids. Current Protocols in Immunology. 33:IV:7.33:7.33.1–7.33.16.

Author Information

  1. Washington University School of Medicine, St. Louis, Missouri

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999
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Abstract

Eicosanoids are arachidonic acid metabolites formed through the cyclooxygenase or one of the lipoxygenase metabolic pathways. They include the prostaglandins (PG), leukotrienes (LT), thromboxanes (Tx), peptidoleukotrienes, and 5-hydroxyeicosatetraenoic acid (5-HETE). This unit describes procedures for measuring individual eicosanoids using several commercial kits. These include kits for an enzyme immunoasssay (ELISA) and a radioimmunoassay (RIA). A method is also described that can be used to analyze the full range of eicosanoids produced in a biological system, and a support protocol presents an acetone/chloroform extraction method for purifying eicosanoids prior to assay. Another support protocol describes a different extraction protocol in which samples are run over an octadecylsilyl silica column.

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