Unit

UNIT 7.33 Measurement of Prostaglandins and Other Eicosanoids

  1. William F. Stenson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0733s33

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Stenson, W. F. 2001. Measurement of Prostaglandins and Other Eicosanoids. Current Protocols in Immunology. 33:IV:7.33:7.33.1–7.33.16.

Author Information

  1. Washington University School of Medicine, St. Louis, Missouri

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999

Abstract

Eicosanoids are arachidonic acid metabolites formed through the cyclooxygenase or one of the lipoxygenase metabolic pathways. They include the prostaglandins (PG), leukotrienes (LT), thromboxanes (Tx), peptidoleukotrienes, and 5-hydroxyeicosatetraenoic acid (5-HETE). This unit describes procedures for measuring individual eicosanoids using several commercial kits. These include kits for an enzyme immunoasssay (ELISA) and a radioimmunoassay (RIA). A method is also described that can be used to analyze the full range of eicosanoids produced in a biological system, and a support protocol presents an acetone/chloroform extraction method for purifying eicosanoids prior to assay. Another support protocol describes a different extraction protocol in which samples are run over an octadecylsilyl silica column.