UNIT 7.33 Measurement of Prostaglandins and Other Eicosanoids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Stenson, W. F. 2001. Measurement of Prostaglandins and Other Eicosanoids. Current Protocols in Immunology. 33:IV:7.33:7.33.1–7.33.16.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
Eicosanoids are arachidonic acid metabolites formed through the cyclooxygenase or one of the lipoxygenase metabolic pathways. They include the prostaglandins (PG), leukotrienes (LT), thromboxanes (Tx), peptidoleukotrienes, and 5-hydroxyeicosatetraenoic acid (5-HETE). This unit describes procedures for measuring individual eicosanoids using several commercial kits. These include kits for an enzyme immunoasssay (ELISA) and a radioimmunoassay (RIA). A method is also described that can be used to analyze the full range of eicosanoids produced in a biological system, and a support protocol presents an acetone/chloroform extraction method for purifying eicosanoids prior to assay. Another support protocol describes a different extraction protocol in which samples are run over an octadecylsilyl silica column.