UNIT 7.35 Assays for B Cell and Germinal Center Development

  1. Jack J.H. Bleesing

Published Online: 1 NOV 2004

DOI: 10.1002/0471142735.im0735s63

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Bleesing, J. J. 2004. Assays for B Cell and Germinal Center Development. Current Protocols in Immunology. 63:IV:7.35:7.35.1–7.35.21.

Author Information

  1. Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2004


The study of B cell development is highly relevant for a better understanding of human disorders in which B cells are involved. B cell development in humans appears to proceed largely along a linear pathway and occurs in three compartments: bone marrow, peripheral blood, and lymphoid tissue. The focus of this unit is on the detailed immunophenotypic evaluation of cell suspensions obtained from these compartments. These protocols are based on routine methodology, commonly used by clinical flow laboratories. Special emphasis will be placed on the approach to the study of B cell development, including current knowledge regarding immunophenotypic identification of B cell subsets. Multiparameter flow cytometry provides powerful analytical tools, as long as attention is paid to careful design and proper execution of flow cytometric acquisition and analysis. The goal of this unit is to provide a guide to the flow cytometric study of B cell development in humans.


  • B cells;
  • B cell development;
  • bone marrow;
  • peripheral blood;
  • lymphoid tissue;
  • germinal center;
  • flow cytometry;
  • immunohistochemistry