UNIT 7.36 In Vitro Models of Human T Cell Anergy

  1. Leonard J. Appleman,
  2. Vassiliki Boussiotis

Published Online: 1 MAR 2005

DOI: 10.1002/0471142735.im0736s65

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Appleman, L. J. and Boussiotis, V. 2005. In Vitro Models of Human T Cell Anergy. Current Protocols in Immunology. 65:IV:7.36:7.36.1–7.36.12.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAR 2005
  2. Published Print: FEB 2005


T cell anergy (acquired unresponsiveness) is an important mechanism for tolerance of self antigens. Human helper T cell clones rendered anergic in vitro are valuable tools for studying the molecular mechanisms of T cell tolerance. This unit outlines the induction and assessment of anergy in human CD4+ helper T cell clones that are reactive against MHC class II alloantigens.