Unit

UNIT 7.41 Sampling of Intestinal Microbiota and Targeted Amplification of Bacterial 16S rRNA Genes for Microbial Ecologic Analysis

  1. Maomeng Tong1,
  2. Jonathan P. Jacobs2,
  3. Ian H. McHardy1,
  4. Jonathan Braun1

Published Online: 3 NOV 2014

DOI: 10.1002/0471142735.im0741s107

Lab Protocol Title

Current Protocols in Immunology

Current Protocols in Immunology

Additional Information

How to Cite

How to cite this article: Curr. Protoc. Immunol. 107:7.41.1–7.41.11. doi: 10.1002/0471142735.im0741s107

Author Information

  1. 1

    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California

  2. 2

    Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, California

Publication History

  1. Published Online: 3 NOV 2014
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Abstract

Dysbiosis of host-associated commensal microbiota is emerging as an important factor in risk and phenotype of immunologic, metabolic, and behavioral diseases. Accurate analysis of microbial composition and functional state in humans or mice requires appropriate collection and pre-processing of biospecimens. Methods to sample luminal and mucosal microbiota from human or mouse intestines and to profile microbial phylogenetic composition using 16S rRNA sequencing are presented here. Data generated using the methods in this unit can be used for downstream quantitative analysis of microbial ecology. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • intestinal microbiota;
  • mucosal microbiota;
  • lavage;
  • Illumina sequencing;
  • 16S rRNA
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