Unit

UNIT 8.1A Solubilization of Lymphocytes

  1. Allan M. Weissman

Published Online: 1 NOV 2003

DOI: 10.1002/0471142735.im0801as57

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Weissman, A. M. 2003. Solubilization of Lymphocytes. Current Protocols in Immunology. 57:8.1A.1–8.1A.9.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 NOV 2003
  2. Published Print: OCT 2003

Abstract

Purification and study of transmembrane proteins require isolation of these structures from their lipid environment. This isolation is carried out through the use of detergents. In this unit, several approaches to solubilization of membrane proteins are presented. Solubilization of whole lymphocytes, using conditions aimed at minimizing the disruption of protein-protein interactions, is described with an optional step that may be useful when the disruption of protein interactions is desired as part of a purification protocol. In some situations, it may be desirable to purify membranes prior to their solubilization or to determine the physical relationship between proteins, which can be accomplished by a cross-linking.