UNIT 8.4 One-Dimensional Gel Electrophoresis of Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gallagher, S. R. and Smith, J. A. 2001. One-Dimensional Gel Electrophoresis of Proteins. Current Protocols in Immunology. 8:III:8.4.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
This is not the most recent version of the article. View current version (1 DEC 2006)
This unit provides several methods for one-dimensional gel electrophoresis of proteins. The basic protocol is the standard method used for gel electrophoresis under denaturing conditions. The first alternate protocol provides conditions used for nondenaturing gel electrophoresis. Gradient gels are described and resolve a much wider molecular-weight range of proteins on a single gel. Minigels, both single-concentration and gradient, are also described.