UNIT 8.4 One-Dimensional Gel Electrophoresis of Proteins

  1. Sean R. Gallagher1,
  2. John A. Smith2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0804s00

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gallagher, S. R. and Smith, J. A. 2001. One-Dimensional Gel Electrophoresis of Proteins. Current Protocols in Immunology. 8:III:8.4.

Author Information

  1. 1

    Hoefer Scientific Instruments, San Francisco, California

  2. 2

    Merck Sharp & Dohme, Rahway, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

This is not the most recent version of the article. View current version (1 DEC 2006)


This unit provides several methods for one-dimensional gel electrophoresis of proteins. The basic protocol is the standard method used for gel electrophoresis under denaturing conditions. The first alternate protocol provides conditions used for nondenaturing gel electrophoresis. Gradient gels are described and resolve a much wider molecular-weight range of proteins on a single gel. Minigels, both single-concentration and gradient, are also described.