Unit
UNIT 8.8 Electroelution of Proteins from Stained Gels
Published Online: 1 FEB 2002
DOI: 10.1002/0471142735.im0808s46
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
Additional Information
How to Cite
Smith, J. A. 2002. Electroelution of Proteins from Stained Gels. Current Protocols in Immunology. 46:III:8.8:8.8.1–8.8.5.
Publication History
- Published Online: 1 FEB 2002
- Published Print: DEC 2001
- Abstract
- Article
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Abstract
In this procedure an SDS-polyacrylamide gel containing electrophoresed proteins is stained with Coomassie brilliant blue, the gel band of interest is excised, and the stained protein is electrophoretically eluted from the minced gel. After removal of most of the salt and sodium dodecyl sulfate (SDS) by dialysis, the protein is precipitated to remove remaining detergent and salt and then analyzed.
