Unit

UNIT 8.8 Electroelution of Proteins from Stained Gels

  1. John A. Smith

Published Online: 1 FEB 2002

DOI: 10.1002/0471142735.im0808s46

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Smith, J. A. 2002. Electroelution of Proteins from Stained Gels. Current Protocols in Immunology. 46:III:8.8:8.8.1–8.8.5.

Author Information

  1. Merck Sharp & Dohme, Rahway, New Jersey

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

Abstract

In this procedure an SDS-polyacrylamide gel containing electrophoresed proteins is stained with Coomassie brilliant blue, the gel band of interest is excised, and the stained protein is electrophoretically eluted from the minced gel. After removal of most of the salt and sodium dodecyl sulfate (SDS) by dialysis, the protein is precipitated to remove remaining detergent and salt and then analyzed.