UNIT 8.11 Iodination of Soluble and Membrane-Bound Proteins

  1. L.E. Samelson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0811s11

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Samelson, L. 2001. Iodination of Soluble and Membrane-Bound Proteins. Current Protocols in Immunology. 11:IV:8.11:8.11.1–8.11.4.

Author Information

  1. National Institute of Child Health and Human Development, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1994


Coupling of radioactive 125I to soluble proteins is a critical step in a number of immunoassays. Chloramine T-catalyzed iodination of soluble proteins, such as immunoglobulins, is described in this unit. Protocols using lactoperoxidase-catalyzed iodination of soluble or membrane-bound proteins are also described.