UNIT 8.11 Iodination of Soluble and Membrane-Bound Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Samelson, L. 2001. Iodination of Soluble and Membrane-Bound Proteins. Current Protocols in Immunology. 11:IV:8.11:8.11.1–8.11.4.
- Published Online: 1 MAY 2001
- Published Print: SEP 1994
Coupling of radioactive 125I to soluble proteins is a critical step in a number of immunoassays. Chloramine T-catalyzed iodination of soluble proteins, such as immunoglobulins, is described in this unit. Protocols using lactoperoxidase-catalyzed iodination of soluble or membrane-bound proteins are also described.