Unit

UNIT 8.12 Biosynthetic Labeling of Proteins

  1. Juan S. Bonifacino

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0812s41

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Bonifacino, J. S. 2001. Biosynthetic Labeling of Proteins. Current Protocols in Immunology. 41:IV:8.12:8.12.1–8.12.9.

Author Information

  1. National Institute of Child Health and Human Development, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001

Abstract

Biosynthetic labeling techniques are commonly used in the study of biochemical properties, synthesis, processing, intracellular transport, secretion, and degradation of proteins. In this unit, protocols are described for biosynthetically labeling many secreted and membrane proteins. The Basic Protocol describes short-term labeling of cells in suspension (30 min to 3 hr) and an alternate protocol describes a modification of this procedure to be used for adherent cells. Alternate protocols for long-term (3 to 24 hr) and pulse-chase labeling of cells are also presented. Label incorporation can be determined as described in a support protocol. The cells used in these protocols can be either from suspension cultures or from single-cell suspensions of spleen or thymus.