UNIT 8.15 Endoglycosidase and Glycoamidase Release of N-Linked Glycans
Published Online: 1 NOV 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Freeze, H. H. and Kranz, C. 2008. Endoglycosidase and Glycoamidase Release of N-Linked Glycans. Current Protocols in Immunology. 83:IV:8.15:8.15.1–8.15.26.
- Published Online: 1 NOV 2008
- Published Print: NOV 2008
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Nearly all proteins entering the lumen of the endoplasmic reticulum (ER) become glycosylated en route to a cellular organelle, the plasma membrane, or the extracellular space. Many glycans can be attached to proteins, but the most common are the N-linked glycans (oligosaccharides). These chains are added very soon after a protein enters the ER, but they undergo extensive remodeling (processing), especially in the Golgi. Processing changes the sensitivity of the N-glycan to enzymes that cleave entire sugar chains or individual monosaccharides, which also changes the migration of the protein on SDS gels. These changes can be used to indicate when a protein has passed a particular subcellular location. This unit details some of the methods used to track a protein as it traffics from the ER to the Golgi toward its final location. Curr. Protoc. Immunol. 83:8.15.1-8.15.26. © 2008 by John Wiley & Sons, Inc.
- intracellular trafficking