UNIT 8.16 Biotinylation and Analysis of Membrane-Bound and Soluble Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Lisanti, M. and Sargiacomo, M. 2001. Biotinylation and Analysis of Membrane-Bound and Soluble Proteins. Current Protocols in Immunology. 36:IV:8.16:8.16.1–8.16.5.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
Coupling of a biotin moiety to proteins allows sensitive detection of those proteins with a variety of commercially available avidin or streptavidin conjugates. This unit presents protocols for biotinylation of cell-surface proteins and proteins in solution. A major advantage of these techniques is that they do not require use of radioisotopes (although use of radioiodinated streptavidin is optional for biotin detection).