Unit

UNIT 8.16 Biotinylation and Analysis of Membrane-Bound and Soluble Proteins

  1. M.P. Lisanti1,
  2. M. Sargiacomo2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0816s36

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lisanti, M. and Sargiacomo, M. 2001. Biotinylation and Analysis of Membrane-Bound and Soluble Proteins. Current Protocols in Immunology. 36:IV:8.16:8.16.1–8.16.5.

Author Information

  1. 1

    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts

  2. 2

    Instituto Superiore di Sanità, Rome, Italy

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000

Abstract

Coupling of a biotin moiety to proteins allows sensitive detection of those proteins with a variety of commercially available avidin or streptavidin conjugates. This unit presents protocols for biotinylation of cell-surface proteins and proteins in solution. A major advantage of these techniques is that they do not require use of radioisotopes (although use of radioiodinated streptavidin is optional for biotin detection).