Unit
UNIT 9.3 Production of Anti-Peptide Antisera
Published Online: 1 AUG 2003
DOI: 10.1002/0471142735.im0903s55
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
Additional Information
How to Cite
Carter, J. M. 2003. Production of Anti-Peptide Antisera. Current Protocols in Immunology. 55:9.3:9.3.1–9.3.16.
Publication History
- Published Online: 1 AUG 2003
- Published Print: JUN 2003
- Abstract
- Article
- References
Abstract
The protocols described in this unit can be used for generating and testing anti-peptide antisera, assuming that a scientist has already selected and conjugated peptides for experimental vaccines. Described are immunization and bleeding of experimental animals, a simple ELISA for antiserum screening, and affinity purification of the serum, including preparation of columns.
Keywords:
- affinity chromatography;
- antibody;
- anti-peptide;
- antiserum;
- ELISA;
- immunization;
- peptide;
- purification;
- vaccine
