Unit

UNIT 9.3 Production of Anti-Peptide Antisera

  1. John Mark Carter

Published Online: 1 AUG 2003

DOI: 10.1002/0471142735.im0903s55

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Carter, J. M. 2003. Production of Anti-Peptide Antisera. Current Protocols in Immunology. 55:9.3:9.3.1–9.3.16.

Author Information

  1. Kensington, California

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUN 2003

Abstract

The protocols described in this unit can be used for generating and testing anti-peptide antisera, assuming that a scientist has already selected and conjugated peptides for experimental vaccines. Described are immunization and bleeding of experimental animals, a simple ELISA for antiserum screening, and affinity purification of the serum, including preparation of columns.

Keywords:

  • affinity chromatography;
  • antibody;
  • anti-peptide;
  • antiserum;
  • ELISA;
  • immunization;
  • peptide;
  • purification;
  • vaccine