UNIT 9.5 Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries

  1. Clemencia Pinilla,
  2. Jon R. Appel,
  3. Richard A. Houghten

Published Online: 1 NOV 2001

DOI: 10.1002/0471142735.im0905s45

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Pinilla, C., Appel, J. R. and Houghten, R. A. 2001. Identification of B Cell and T Cell Epitopes Using Synthetic Peptide Combinatorial Libraries. Current Protocols in Immunology. 65:9.5:9.5.1–9.5.13.

Author Information

  1. Torrey Pines Institute for Molecular Studies, San Diego, California

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: OCT 2001

This is not the most recent version of the article. View current version (1 NOV 2012)


This unit presents a combinatorial library method that consists of the synthesis and screening of mixture-based synthetic combinatorial libraries (SCL) of peptide molecules. The protocols employ peptide SCL to identify peptides recognized by mAbs and T cells. The first protocol uses a positional scanning SCL (PS-SCL) made up of hexapeptides to identify antigenic determinants recognized by mAbs. The 120 mixtures in the hexapeptide PS-SCL are tested for their inhibitory activity in a competitive ELISA. The second protocol uses a decapeptide PS-SCL to identify T cell peptide ligands. The 200 mixtures of the decapeptide PS-SCL are tested for their ability to induce proliferation. Support protocols cover optimization of the assay conditions for each mAb or T cell, to achieve the best level of sensitivity and reproducibility, and preparation of a hexapeptide PS-SCL, along with deconvolution approaches.