Unit

UNIT 9.8 Phage Display Selection, Analysis, and Prediction of B Cell Epitopes

  1. Natalia Tarnovitski Freund1,
  2. David Enshell-Seijffers2,
  3. Jonathan M. Gershoni1

Published Online: 1 AUG 2009

DOI: 10.1002/0471142735.im0908s86

Lab Protocol Title

Current Protocols in Immunology

Current Protocols in Immunology

Additional Information

How to Cite

Freund, N. T., Enshell-Seijffers, D. and Gershoni, J. M. 2009. Phage Display Selection, Analysis, and Prediction of B Cell Epitopes. Current Protocols in Immunology. 86:9.8:9.8.1–9.8.30.

Author Information

  1. 1

    Tel Aviv University, Tel Aviv, Israel

  2. 2

    Harvard Medical School, Charlestown, Massachusetts

Publication History

  1. Published Online: 1 AUG 2009
  2. Published Print: AUG 2009
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Abstract

Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • epitope mapping;
  • mimotope;
  • peptide mimetics;
  • phage display;
  • random peptide libraries;
  • Mapitope;
  • Pepitope
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