UNIT 9.8 Phage Display Selection, Analysis, and Prediction of B Cell Epitopes
Published Online: 1 AUG 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Freund, N. T., Enshell-Seijffers, D. and Gershoni, J. M. 2009. Phage Display Selection, Analysis, and Prediction of B Cell Epitopes. Current Protocols in Immunology. 86:9.8:9.8.1–9.8.30.
- Published Online: 1 AUG 2009
- Published Print: AUG 2009
Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed “biopanning.” The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels of MAb-specific peptides allow computational prediction of B cell epitopes. Epitope profiles recognized by polyclonal serum samples can also be generated. Detailed step by step protocols and discussion of applications are provided. Curr. Protoc. Immunol. 86:9.8.1-9.8.30. © 2009 by John Wiley & Sons, Inc.
- epitope mapping;
- peptide mimetics;
- phage display;
- random peptide libraries;