UNIT 10.2 Preparation of Genomic DNA from Mammalian Tissue
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Strauss, W. 2001. Preparation of Genomic DNA from Mammalian Tissue. Current Protocols in Immunology. 8:I:10.2:10.2.1–10.2.3.
- Published Online: 1 MAY 2001
- Published Print: DEC 1993
There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteinization and recovery of DNA. The main differences between various approaches lie in the extent of deproteinization and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.