Unit

UNIT 10.2 Preparation of Genomic DNA from Mammalian Tissue

  1. William.M. Strauss

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1002s08

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Strauss, W. 2001. Preparation of Genomic DNA from Mammalian Tissue. Current Protocols in Immunology. 8:I:10.2:10.2.1–10.2.3.

Author Information

  1. Whitehead Institute, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1993

Abstract

There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteinization and recovery of DNA. The main differences between various approaches lie in the extent of deproteinization and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.