Unit

UNIT 10.7 Deprotection of Oligonucleotides and Purification Using Denaturing PAGE

  1. Andrew Ellington1,
  2. Joanne Chory (PAGE setup)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1007s06

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ellington, A. and Chory, J. 2001. Deprotection of Oligonucleotides and Purification Using Denaturing PAGE. Current Protocols in Immunology. 2:III:10.7:10.7.1–10.7.6.

Author Information

  1. 1

    Indiana University, Bloomington, Indiana

  2. 2

    The Salk Institute, La Jolla, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1993

Abstract

The advantages of purification by denaturing polyacrylamide gel electrophoresis (PAGE) are speed, simplicity, and high resolution. Although yields tend to be low (<50% of applied sample), the amount of material recovered is usually far in excess of that required for most molecular biology applications (e.g., cloning and sequencing). Denaturing PAGE can resolve oligonucleotides from 2 to 300 bases, depending on the percentage of polyacrylamide used. The method presented here is thus useful not only for isolating chemically synthesized deoxyribonucleotides but also small RNAs or other single-stranded polynucleotides.