UNIT 10.7 Deprotection of Oligonucleotides and Purification Using Denaturing PAGE
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Ellington, A. and Chory, J. 2001. Deprotection of Oligonucleotides and Purification Using Denaturing PAGE. Current Protocols in Immunology. 2:III:10.7:10.7.1–10.7.6.
- Published Online: 1 MAY 2001
- Published Print: JUN 1993
The advantages of purification by denaturing polyacrylamide gel electrophoresis (PAGE) are speed, simplicity, and high resolution. Although yields tend to be low (<50% of applied sample), the amount of material recovered is usually far in excess of that required for most molecular biology applications (e.g., cloning and sequencing). Denaturing PAGE can resolve oligonucleotides from 2 to 300 bases, depending on the percentage of polyacrylamide used. The method presented here is thus useful not only for isolating chemically synthesized deoxyribonucleotides but also small RNAs or other single-stranded polynucleotides.