UNIT 10.8 Digestion of DNA with Restriction Endonucleases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Bloch, K. D. 2001. Digestion of DNA with Restriction Endonucleases. Current Protocols in Immunology. 2:IV:10.8:10.8.1–10.8.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 1992
Restriction endonucleases recognize short DNA sequences and catalyze the cleavage of double-stranded DNA at specific sites within or adjacent to the recognition sequences. This unit describes how to cleave DNA and refers the investigator to frequently updated manuals by endonuclease suppliers for the precise optimized conditions. Alternate procedures are given for digesting a DNA sample with several different enzymes and digesting multiple DNA samples with the same endonuclease.