UNIT 10.8 Digestion of DNA with Restriction Endonucleases

  1. Kenneth D. Bloch

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1008s02

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Bloch, K. D. 2001. Digestion of DNA with Restriction Endonucleases. Current Protocols in Immunology. 2:IV:10.8:10.8.1–10.8.7.

Author Information

  1. Massachusetts General Hospital, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1992


Restriction endonucleases recognize short DNA sequences and catalyze the cleavage of double-stranded DNA at specific sites within or adjacent to the recognition sequences. This unit describes how to cleave DNA and refers the investigator to frequently updated manuals by endonuclease suppliers for the precise optimized conditions. Alternate procedures are given for digesting a DNA sample with several different enzymes and digesting multiple DNA samples with the same endonuclease.