Unit

UNIT 10.9 Reagents and Radioisotopes Used to Manipulate Nucleic Acids

  1. Kevin Struhl

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1009s02

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Struhl, K. 2001. Reagents and Radioisotopes Used to Manipulate Nucleic Acids. Current Protocols in Immunology. 2:IV:10.9:10.9.1–10.9.9.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1992

Abstract

The application of molecular biological techniques to immunological studies is based in large part on the manipulation of nucleic acids--RNA, DNA, and oligonucleotides. This unit offers very basic procedures that are used in a number of protocols later in the chapter. In particular, preparation of nucleoside triphosphates--the substrates for almost all synthetic reactions using nucleic acids--is described; the relevant radioisotopes, 32P, 35S, and 3H are discussed; and methods for quantitating radioisotope and biotin incorporation into nucleic acids are presented. Finally, column chromatographic (gel filtration) procedures for separating labeled DNA from synthetic precursors are described.