UNIT 10.9 Reagents and Radioisotopes Used to Manipulate Nucleic Acids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Struhl, K. 2001. Reagents and Radioisotopes Used to Manipulate Nucleic Acids. Current Protocols in Immunology. 2:IV:10.9:10.9.1–10.9.9.
- Published Online: 1 MAY 2001
- Published Print: JUN 1992
The application of molecular biological techniques to immunological studies is based in large part on the manipulation of nucleic acids--RNA, DNA, and oligonucleotides. This unit offers very basic procedures that are used in a number of protocols later in the chapter. In particular, preparation of nucleoside triphosphates--the substrates for almost all synthetic reactions using nucleic acids--is described; the relevant radioisotopes, 32P, 35S, and 3H are discussed; and methods for quantitating radioisotope and biotin incorporation into nucleic acids are presented. Finally, column chromatographic (gel filtration) procedures for separating labeled DNA from synthetic precursors are described.