UNIT 10.11 Preparation of RNA from Tissues and Cells

  1. Randall Ribaudo (hot phenol method)1,
  2. Michael Gilman (cytoplasmic RNA isolation and removal of contaminating DNA)2,
  3. Robert E. Kingston (CsCl isolation and poly(A) selection)3,
  4. Piotr Choczynski (single-step isolation)4,
  5. Nicoletta Sacchi (single-step isolation)5

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1011s04

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ribaudo, R., Gilman, M., Kingston, R. E., Choczynski, P. and Sacchi, N. 2001. Preparation of RNA from Tissues and Cells. Current Protocols in Immunology. 3:V:10.11:10.11.1–10.11.14.

Author Information

  1. 1

    National Institute of Allergy and Infectious Disease, Bethesda, Maryland

  2. 2

    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

  3. 3

    Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

  4. 4

    University of Cincinnati College of Medicine, Cincinnati, Ohio

  5. 5

    Life Technologies, Gaithersburg, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1992

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