Unit

UNIT 10.14 Transfection Using DEAE-Dextran

  1. Richard F Selden

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1014s03

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Selden, R. F. 2001. Transfection Using DEAE-Dextran. Current Protocols in Immunology. 3:VI:10.14:10.14.1–10.14.6.

Author Information

  1. Transkaryotic Therapies, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1992

Abstract

Two protocols for DEAE-dextran transfection of cells are provided in this unit. The Basic Protocol describes a procedure used to transfect adherent cells and the first Alternate Protocol presents a method used to transfect suspension cells. If an increase in transfection efficiency is needed, cells can be treated with chloroquine as described in the second Alternate Protocol.