UNIT 10.14 Transfection Using DEAE-Dextran
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Selden, R. F. 2001. Transfection Using DEAE-Dextran. Current Protocols in Immunology. 3:10.14.1–10.14.6.
- Published Online: 1 MAY 2001
- Published Print: SEP 1992
Two protocols for DEAE-dextran transfection of cells are provided in this unit. The Basic Protocol describes a procedure used to transfect adherent cells and the first Alternate Protocol presents a method used to transfect suspension cells. If an increase in transfection efficiency is needed, cells can be treated with chloroquine as described in the second Alternate Protocol.