UNIT 10.16 Liposome-Mediated Transfection

  1. Michael Whitt1,
  2. Linda Buonocore2,
  3. John K. Rose2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1016s03

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Whitt, M., Buonocore, L. and Rose, J. K. 2001. Liposome-Mediated Transfection. Current Protocols in Immunology. 3:VI:10.16:10.16.1–10.16.4.

Author Information

  1. 1

    University of Tennessee, Memphis, Tennessee

  2. 2

    Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1992


Using liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the Basic Protocol describes a transient expression system while an Alternate Protocol involves stable transformation and expression of DNA integrated into the genome of the transfected cell. In both protocols, plasmid DNA derived from either crude (miniprep) or purified (through CsCl) preparations is mixed with a liposome suspension comprised of cationic lipids and applied to monolayer cell cultures.