UNIT 10.16 Liposome-Mediated Transfection
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Whitt, M., Buonocore, L. and Rose, J. K. 2001. Liposome-Mediated Transfection. Current Protocols in Immunology. 3:VI:10.16:10.16.1–10.16.4.
- Published Online: 1 MAY 2001
- Published Print: SEP 1992
Using liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the Basic Protocol describes a transient expression system while an Alternate Protocol involves stable transformation and expression of DNA integrated into the genome of the transfected cell. In both protocols, plasmid DNA derived from either crude (miniprep) or purified (through CsCl) preparations is mixed with a liposome suspension comprised of cationic lipids and applied to monolayer cell cultures.