Unit

UNIT 10.17B Transfection of Lymphoid Cells

  1. Sherie L. Morrison

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1017bs31

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Morrison, S. L. 2001. Transfection of Lymphoid Cells. Current Protocols in Immunology. 12:VI:10.17B:10.17.7–10.17.13.

Author Information

  1. University of California Los Angeles, Los Angeles, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1999

Abstract

Using available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The Basic Protocol in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more cumbersome than electroporation or liposome-mediated transfection, which are described in the two alternate protocols. All three techniques may be adapted for stable transfection of other eukaryotic cell lines.