UNIT 10.17B Transfection of Lymphoid Cells
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Morrison, S. L. 2001. Transfection of Lymphoid Cells. Current Protocols in Immunology. 12:VI:10.17B:10.17.7–10.17.13.
- Published Online: 1 MAY 2001
- Published Print: JUN 1999
Using available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The Basic Protocol in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more cumbersome than electroporation or liposome-mediated transfection, which are described in the two alternate protocols. All three techniques may be adapted for stable transfection of other eukaryotic cell lines.