Unit

UNIT 10.17D Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro

  1. Yoshitaka Kondo,
  2. Keizo Miyata,
  3. Fuminori Kato

Published Online: 1 APR 2010

DOI: 10.1002/0471142735.im1017ds89

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kondo, Y., Miyata, K. and Kato, F. 2010. Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro. Current Protocols in Immunology. 89:VI:10.17D:10.17D.1–10.17D.9.

Author Information

  1. Life Science Research Laboratory, Central Research Institute, Ishihara Sangyo Kaisha, Ltd., Kusatsu, Shiga, Japan

Publication History

  1. Published Online: 1 APR 2010
  2. Published Print: APR 2010

Abstract

The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non-viral vector. Because replication and transcription of genomic RNA is inactivated by β-propiolactone treatment or UV-irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope. Curr. Protoc. Immunol. 89:10.17D.1-10.17D.9. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • hemagglutinating virus of Japan;
  • Sendai virus;
  • HVJ envelope;
  • siRNA transfection