UNIT 10.17D Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro
Published Online: 1 APR 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kondo, Y., Miyata, K. and Kato, F. 2010. Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro. Current Protocols in Immunology. 89:VI:10.17D:10.17D.1–10.17D.9.
- Published Online: 1 APR 2010
- Published Print: APR 2010
The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non-viral vector. Because replication and transcription of genomic RNA is inactivated by β-propiolactone treatment or UV-irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope. Curr. Protoc. Immunol. 89:10.17D.1-10.17D.9. © 2010 by John Wiley & Sons, Inc.
- hemagglutinating virus of Japan;
- Sendai virus;
- HVJ envelope;
- siRNA transfection