Unit

UNIT 10.17E Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models

  1. Atsushi Kitani1,
  2. Stefan Fichtner-Feigl2

Published Online: 1 NOV 2010

DOI: 10.1002/0471142735.im1017es91

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kitani, A. and Fichtner-Feigl, S. 2010. Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models. Current Protocols in Immunology. 91:VI:10.17E:10.17E.1–10.17E.9.

Author Information

  1. 1

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    University of Regensburg, Regensburg, Germany

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010

Abstract

This unit describes a method for in vivo delivery of oligonucleotides or plasmids using the hemagglutinating virus of Japan envelope (HVJ-E), an inactivated Sendai virus particle, as a delivery system. Viral transfection methods generally show a higher transfection efficiency than nonviral methods for the delivery of genes to cells. However, in using these methods one must bear in mind that the introduction of a virus particle into a host carries a risk for leukemia induction and for creation of disturbances in immune function due to cytotoxicity. Curr. Protoc. Immunol. 91:10.17E.1-10.17E.9. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • hemagglutinating virus of Japan;
  • Sendai virus;
  • plasmid;
  • siRNA;
  • decoy oligo;
  • gene therapy;
  • in vivo