UNIT 10.17E Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kitani, A. and Fichtner-Feigl, S. 2010. Efficient In Vivo Delivery of Plasmids and Oligonucleotides Using Hemagglutinating Virus of Japan Envelope (HVJ-E) Vector in Immunological Disease Models. Current Protocols in Immunology. 91:VI:10.17E:10.17E.1–10.17E.9.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
This unit describes a method for in vivo delivery of oligonucleotides or plasmids using the hemagglutinating virus of Japan envelope (HVJ-E), an inactivated Sendai virus particle, as a delivery system. Viral transfection methods generally show a higher transfection efficiency than nonviral methods for the delivery of genes to cells. However, in using these methods one must bear in mind that the introduction of a virus particle into a host carries a risk for leukemia induction and for creation of disturbances in immune function due to cytotoxicity. Curr. Protoc. Immunol. 91:10.17E.1-10.17E.9. © 2010 by John Wiley & Sons, Inc.
- hemagglutinating virus of Japan;
- Sendai virus;
- decoy oligo;
- gene therapy;
- in vivo