Unit

UNIT 10.18 Use of Monoclonal Antibodies for Expression Cloning

  1. Diane Hollenbaugh,
  2. Alejandro Aruffo (cell-surface and intracellular antigens),
  3. Bryan Jones,
  4. Peter Linsley (intracellular antigens)

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1018s31

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hollenbaugh, D., Aruffo, A., Jones, B. and Linsley, P. 2001. Use of Monoclonal Antibodies for Expression Cloning. Current Protocols in Immunology. 31:VI:10.18:10.18.1–10.18.16.

Author Information

  1. Bristol-Myers Squibb, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1999

Abstract

This unit details the use of transient expression in mammalian cells to screen cDNA libraries with monoclonal antibodies (MAb) to isolate cDNA clones encoding cell-surface and intracellular proteins. The first protocol describes the cloning of cDNAs encoding cell-surface antigens. The second protocol is a modification that facilitates isolation of cDNAs encoding antigens that are expressed intracellularly. Both protocols are designed for use with the expression vector CDM8, which contains a polylinker for subcloning double-stranded cDNA.