UNIT 10.19A Construction of Immunoglobulin Fusion Proteins
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Hollenbaugh, D. and Aruffo, A. 2002. Construction of Immunoglobulin Fusion Proteins. Current Protocols in Immunology. 48:VI:10.19A:10.19A.1–10.19A.11.
- Published Online: 1 MAY 2002
- Published Print: APR 2002
Recombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a modified version of the expression vector pCDM8. The resulting fusion protein generally retains the functional properties of both the protein of interest and the immunoglobulin constant region; this can be demonstrated as described here.