Unit

UNIT 10.19A Construction of Immunoglobulin Fusion Proteins

  1. Diane Hollenbaugh,
  2. Alejandro Aruffo

Published Online: 1 MAY 2002

DOI: 10.1002/0471142735.im1019as48

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hollenbaugh, D. and Aruffo, A. 2002. Construction of Immunoglobulin Fusion Proteins. Current Protocols in Immunology. 48:VI:10.19A:10.19A.1–10.19A.11.

Author Information

  1. Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: APR 2002

Abstract

Recombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a modified version of the expression vector pCDM8. The resulting fusion protein generally retains the functional properties of both the protein of interest and the immunoglobulin constant region; this can be demonstrated as described here.