UNIT 10.26 Evaluating Human T Cell Receptor Gene Expression by PCR
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gussoni, E., Panzara, M. A. and Steinman, L. 2001. Evaluating Human T Cell Receptor Gene Expression by PCR. Current Protocols in Immunology. 22:VII:10.26:10.26.1–10.26.14.
- Published Online: 1 MAY 2001
- Published Print: JUN 1997
This unit describes the use of PCR to characterize and quantify rearranged transcripts from specific T cell receptor variable gene families in human tissue and peripheral blood lymphocytes. The strategy outlined in this unit has been extensively used on different sources of human tissue including brain, spinal cord, and skeletal muscle. A protocol is provided to clone and sequence PCR-amplified cDNA transcripts to study the junctional diversity of the expressed genes. A support protocol describes a method for reverse transcribing total RNA to make the cDNA required by the other protocols.