UNIT 10.26 Evaluating Human T Cell Receptor Gene Expression by PCR

  1. Emanuela Gussoni1,
  2. Michael A. Panzara2,
  3. Lawrence Steinman3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1026s22

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gussoni, E., Panzara, M. A. and Steinman, L. 2001. Evaluating Human T Cell Receptor Gene Expression by PCR. Current Protocols in Immunology. 22:VII:10.26:10.26.1–10.26.14.

Author Information

  1. 1

    Children's Hospital, Boston, Massachusetts

  2. 2

    Massachusetts General Hospital, Boston, Massachusetts

  3. 3

    Stanford University School of Medicine, Stanford, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1997


This unit describes the use of PCR to characterize and quantify rearranged transcripts from specific T cell receptor variable gene families in human tissue and peripheral blood lymphocytes. The strategy outlined in this unit has been extensively used on different sources of human tissue including brain, spinal cord, and skeletal muscle. A protocol is provided to clone and sequence PCR-amplified cDNA transcripts to study the junctional diversity of the expressed genes. A support protocol describes a method for reverse transcribing total RNA to make the cDNA required by the other protocols.