Unit

UNIT 10.29 Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression

  1. Howard A. Young,
  2. Jeffrey J. Subleski,
  3. Stephanie M. Krebs

Published Online: 1 AUG 2003

DOI: 10.1002/0471142735.im1029s54

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Young, H. A., Subleski, J. J. and Krebs, S. M. 2003. Multiprobe Ribonuclease Protection Assay for Simultaneous Measurement of mRNA Expression. Current Protocols in Immunology. 54:VII:10.29:10.29.1–10.29.15.

Author Information

  1. National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: APR 2003

Abstract

The multiprobe RNase protection assay enables investigators to monitor RNA expression of 8-12 genes with as little as 1 µg of total RNA. The commercial availability of numerous multi-gene template sets makes this assay practical for all basic research programs.