UNIT 10.30 Analysis of Telomere Length and Telomerase Activity
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Hathcock, K. S., Hodes, R. J. and Weng, N.-P. 2004. Analysis of Telomere Length and Telomerase Activity. Current Protocols in Immunology. 62:VII:10.30:10.30.1–10.30.27.
- Published Online: 1 SEP 2004
- Published Print: AUG 2004
Telomeres are specialized DNA-protein structures present at the ends of all linear chromosomes and are characterized by (TTAGGG)n hexanucleotide repeats and associated proteins. Telomere length has been implicated in cell survival and replicative capacity of dividing somatic cells. In the absence of an active compensatory mechanism, telomere lengths shorten as a consequence of proliferation, both in vitro and in vivo. However, this loss of telomeric repeats can be compensated and telomere length maintained by the enzyme telomerase, which is capable of adding (TTAGGG) repeat sequences to the ends of telomeres. This unit describes methods that are used for the measurement of telomere length and telomerase activity in human and murine cells.
- hexanucleotide repeates;
- cell senescence;