Unit

UNIT 10.33 Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR

  1. Máire F. Quigley1,
  2. Jorge R. Almeida1,
  3. David A. Price1,2,
  4. Daniel C. Douek1

Published Online: 1 AUG 2011

DOI: 10.1002/0471142735.im1033s94

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Quigley, M. F., Almeida, J. R., Price, D. A. and Douek, D. C. 2011. Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR. Current Protocols in Immunology. 94:VII:10.33:10.33.1–10.33.16.

Author Information

  1. 1

    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

  2. 2

    Department of Infection, Immunity, and Biochemistry, Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom

Publication History

  1. Published Online: 1 AUG 2011
  2. Published Print: AUG 2011

Abstract

A detailed knowledge of the principles that guide clonal selection within the memory and effector T cell pools is essential to further our understanding of the factors that influence effective T cell-mediated immunity and has direct implications for the rational design of vaccines and immunotherapies. This unit provides methods for the unbiased quantification and characterization of all expressed T cell receptor (TCR) gene products within any defined T cell population. The approach is based on a template-switch anchored reverse transcription–polymerase chain reaction (RT-PCR) and is optimized for the analysis of antigen-specific T cells isolated directly ex vivo. Curr. Protoc. Immunol. 94:10.33.1-10.33.16. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • T cell receptor;
  • polymerase chain reaction;
  • antigen-specific T cell