UNIT 10.33 Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR
Published Online: 1 AUG 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Quigley, M. F., Almeida, J. R., Price, D. A. and Douek, D. C. 2011. Unbiased Molecular Analysis of T Cell Receptor Expression Using Template-Switch Anchored RT-PCR. Current Protocols in Immunology. 94:VII:10.33:10.33.1–10.33.16.
- Published Online: 1 AUG 2011
- Published Print: AUG 2011
A detailed knowledge of the principles that guide clonal selection within the memory and effector T cell pools is essential to further our understanding of the factors that influence effective T cell-mediated immunity and has direct implications for the rational design of vaccines and immunotherapies. This unit provides methods for the unbiased quantification and characterization of all expressed T cell receptor (TCR) gene products within any defined T cell population. The approach is based on a template-switch anchored reverse transcription–polymerase chain reaction (RT-PCR) and is optimized for the analysis of antigen-specific T cells isolated directly ex vivo. Curr. Protoc. Immunol. 94:10.33.1-10.33.16. © 2011 by John Wiley & Sons, Inc.
- T cell receptor;
- polymerase chain reaction;
- antigen-specific T cell