UNIT 10.34 Immunologic Applications of Conditional Gene Modification Technology in the Mouse
Published Online: 2 APR 2014
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Sharma, S. and Zhu, J. 2014. Immunologic Applications of Conditional Gene Modification Technology in the Mouse. Current Protocols in Immunology. 105:VIII:10.34:10.34.1–10.34.13.
- Published Online: 2 APR 2014
Since the success of homologous recombination in altering mouse genome and the discovery of Cre-loxP system, the combination of these two breakthroughs has created important applications for studying the immune system in the mouse. Here, we briefly summarize the general principles of this technology and its applications in studying immune cell development and responses; such implications include conditional gene knockout and inducible and/or tissue-specific gene over-expression, as well as lineage fate mapping. We then discuss the pros and cons of a few commonly used Cre-expressing mouse lines for studying lymphocyte development and functions. We also raise several general issues, such as efficiency of gene deletion, leaky activity of Cre, and Cre toxicity, all of which may have profound impacts on data interpretation. Finally, we selectively list some useful links to the Web sites as valuable mouse resources. Curr. Protoc. Immunol. 105:10.34.1-10.34.13. © 2014 by John Wiley & Sons, Inc.
- conditional knockout;
- bacterial artificial chromosome (BAC);
- hematopoietic cells;
- fate mapping;
- inducible gene expression;
- lymphocyte cell development and differentiation