Unit

UNIT 11.5 Assessment of the Effects of Tyrosine Protein Kinase Inhibitors

  1. Anne L. Burkhardt,
  2. Joseph B. Bolen

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1105s07

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Burkhardt, A. L. and Bolen, J. B. 2001. Assessment of the Effects of Tyrosine Protein Kinase Inhibitors. Current Protocols in Immunology. 7:11.5:11.5.1–11.5.6.

Author Information

  1. Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1993

Abstract

The tyrosine protein kinases are enzymes that are important in cellular signal transduction. Therefore, inhibition of TPKs provides an important means of investigating and potentially controlling many signaling pathways. The first basic protocol in this unit describes an assay of the inhibitory effects of TPK inhibitors in vitro on a specific TPK that has been immune-precipitated from cell lysates. An assay of the effects of several TPK inhibitors on TPKs in vivo in activated cells is also provided. Although the example used here is a nonreceptor TPK, these protocols can be used to assay the effects of inhibitors on receptor TPKs as well.