Unit

UNIT 11.8 Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells

  1. Alan J. Whitmarsh1,
  2. Roger J. Davis2

Published Online: 1 FEB 2004

DOI: 10.1002/0471142735.im1108s58

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Whitmarsh, A. J. and Davis, R. J. 2004. Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells. Current Protocols in Immunology. 58:11.8:11.8.1–11.8.17.

Author Information

  1. 1

    University of Manchester, School of Biological Sciences, Manchester, United Kingdom

  2. 2

    University of Massachusetts Medical School, Worcester, Massachusetts

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: DEC 2003

Abstract

The recognition of peptide antigens by T cells through their antigen receptors (T cell receptors, or TCR), together with the ligation of additional surface molecules called costimulatory receptors, rapidly induces interactive signaling pathways that lead to transcriptional initiation at genes such as that of the autocrine growth factor interleukin 2 (IL-2). Activation of the ERK and JNK subfamilies of MAPK mediates some of these signals. This unit presents procedures for a solid-phase kinase assay and immune-complex kinase assay to measure JNK and ERK activities, respectively, in T cells that have been appropriately stimulated. Also described is a procedure for preparing GSThyphen;cJun/GSH-Sepharose beads needed in the solid-phase JNK protein kinase activity assay.