UNIT 11.8 Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Whitmarsh, A. J. and Davis, R. J. 2004. Analyzing Mitogen-Activated Protein Kinase (MAPK) Activities in T Cells. Current Protocols in Immunology. 58:11.8:11.8.1–11.8.17.
- Published Online: 1 FEB 2004
- Published Print: DEC 2003
The recognition of peptide antigens by T cells through their antigen receptors (T cell receptors, or TCR), together with the ligation of additional surface molecules called costimulatory receptors, rapidly induces interactive signaling pathways that lead to transcriptional initiation at genes such as that of the autocrine growth factor interleukin 2 (IL-2). Activation of the ERK and JNK subfamilies of MAPK mediates some of these signals. This unit presents procedures for a solid-phase kinase assay and immune-complex kinase assay to measure JNK and ERK activities, respectively, in T cells that have been appropriately stimulated. Also described is a procedure for preparing GSThyphen;cJun/GSH-Sepharose beads needed in the solid-phase JNK protein kinase activity assay.