UNIT 12.3 Infection of CD4+ Primary Cell Lines and Induction of HIV
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Poli, G. and Fauci, A. S. 2001. Infection of CD4+ Primary Cell Lines and Induction of HIV. Current Protocols in Immunology. 12:12.3.
- Published Online: 1 MAY 2001
- Published Print: MAR 1993
This is not the most recent version of the article. View current version (1 NOV 2005)
Chronically infected cell lines and clones are currently used as sources of virus to be used to infect other cell types as “factories” for large-scale production of virus or viral components and as model systems for studies of the regulation of virus expression. In this unit, conditions for acute in vitro HIV infection are described. “Survivor” cells, resistant to the cytopathic effect of the virus, can be obtained from acute cultures. These survivor cells maintain an intact proliferative capacity and are considered to be chronically infected; their generation is described here. Finally, it is often desirable to study the effects of stimulants on HIV induction in these chronically infected cell lines; this procedure is detailed in a third protocol.