UNIT 12.4 Culture of HIV in Monocytes and Macrophages

  1. Suzanne Crowe1,
  2. Clare Maslin1,
  3. Philip Ellery1,
  4. Katherine Kedzierska2

Published Online: 1 SEP 2004

DOI: 10.1002/0471142735.im1204s62

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Crowe, S., Maslin, C., Ellery, P. and Kedzierska, K. 2004. Culture of HIV in Monocytes and Macrophages. Current Protocols in Immunology. 62:12.4:12.4.1–12.4.13.

Author Information

  1. 1

    Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia

  2. 2

    University of Melbourne, Melbourne, Australia

Publication History

  1. Published Online: 1 SEP 2004
  2. Published Print: AUG 2004

This is not the most recent version of the article. View current version (1 JAN 2006)


Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks.