UNIT 12.4 Culture of HIV in Monocytes and Macrophages
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Crowe, S., Maslin, C., Ellery, P. and Kedzierska, K. 2004. Culture of HIV in Monocytes and Macrophages. Current Protocols in Immunology. 62:12.4:12.4.1–12.4.13.
- Published Online: 1 SEP 2004
- Published Print: AUG 2004
This is not the most recent version of the article. View current version (1 JAN 2006)
Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks.